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	<title>Comments on: Epitopes and Microsoft Computational Biology</title>
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	<link>http://www.iayork.com/MysteryRays/2007/06/14/epitopes-and-microsoft-computational-biology/</link>
	<description>Meddling with things mankind is not meant to understand.  Also, pictures of my kids</description>
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		<title>By: Sebastin</title>
		<link>http://www.iayork.com/MysteryRays/2007/06/14/epitopes-and-microsoft-computational-biology/comment-page-1/#comment-10840</link>
		<dc:creator>Sebastin</dc:creator>
		<pubDate>Fri, 12 Dec 2008 07:28:01 +0000</pubDate>
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		<description>can you help list some new online epitope prediction tools or link us or post a new post on this. Much appreciated!</description>
		<content:encoded><![CDATA[<p>can you help list some new online epitope prediction tools or link us or post a new post on this. Much appreciated!</p>
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		<title>By: Mystery Rays from Outer Space - Meddling with things mankind is not meant to understand. Also, pictures of my kids &#187; Epitope prediction: The seven percent solution</title>
		<link>http://www.iayork.com/MysteryRays/2007/06/14/epitopes-and-microsoft-computational-biology/comment-page-1/#comment-109</link>
		<dc:creator>Mystery Rays from Outer Space - Meddling with things mankind is not meant to understand. Also, pictures of my kids &#187; Epitope prediction: The seven percent solution</dc:creator>
		<pubDate>Fri, 28 Sep 2007 03:16:23 +0000</pubDate>
		<guid isPermaLink="false">http://www.iayork.com/MysteryRays/2007/06/14/epitopes-and-microsoft-computational-biology/#comment-109</guid>
		<description>[...] prediction: The seven percent solution   By iayork  I&#8217;ve talked several times (for example, here, here, and here) about predicting cytotoxic T lymphocyte (CTL) epitopes, and emphasized how hard it [...]</description>
		<content:encoded><![CDATA[<p>[...] prediction: The seven percent solution   By iayork  I&#8217;ve talked several times (for example, here, here, and here) about predicting cytotoxic T lymphocyte (CTL) epitopes, and emphasized how hard it [...]</p>
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		<title>By: Mystery Rays from Outer Space - Meddling with things mankind is not meant to understand. Also, pictures of my kids &#187; Epitope prediction: The bad and the ugly</title>
		<link>http://www.iayork.com/MysteryRays/2007/06/14/epitopes-and-microsoft-computational-biology/comment-page-1/#comment-57</link>
		<dc:creator>Mystery Rays from Outer Space - Meddling with things mankind is not meant to understand. Also, pictures of my kids &#187; Epitope prediction: The bad and the ugly</dc:creator>
		<pubDate>Thu, 23 Aug 2007 17:59:54 +0000</pubDate>
		<guid isPermaLink="false">http://www.iayork.com/MysteryRays/2007/06/14/epitopes-and-microsoft-computational-biology/#comment-57</guid>
		<description>[...] prediction: The bad and the ugly   By iayork When I was talking about Microsoft&#8217;s epitope prediction software, and when I discussed Kotturi&#8217;s update on LCMV [...]</description>
		<content:encoded><![CDATA[<p>[...] prediction: The bad and the ugly   By iayork When I was talking about Microsoft&#8217;s epitope prediction software, and when I discussed Kotturi&#8217;s update on LCMV [...]</p>
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		<title>By: iayork</title>
		<link>http://www.iayork.com/MysteryRays/2007/06/14/epitopes-and-microsoft-computational-biology/comment-page-1/#comment-28</link>
		<dc:creator>iayork</dc:creator>
		<pubDate>Wed, 01 Aug 2007 19:38:24 +0000</pubDate>
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		<description>You&#039;re right, it&#039;s gag/pol, not nef.   

I didn&#039;t note down the accession number at the time.  From my databases it could have been any of 
P03366
P05959
P04585
P03368
P05961
P35963
P12499
K02013 

The last (K02013, Human immunodeficiency virus type 1, isolate BRU, complete genome (LAV-1)) is the most likely, because all the rest specifically say &quot;Gag-Pol polyprotein (Pr160Gag-Pol)&quot;.  I think I grabbed the sequence and was in too much rush when I tried to work out which protein I was dealing with.</description>
		<content:encoded><![CDATA[<p>You&#8217;re right, it&#8217;s gag/pol, not nef.   </p>
<p>I didn&#8217;t note down the accession number at the time.  From my databases it could have been any of<br />
P03366<br />
P05959<br />
P04585<br />
P03368<br />
P05961<br />
P35963<br />
P12499<br />
K02013 </p>
<p>The last (K02013, Human immunodeficiency virus type 1, isolate BRU, complete genome (LAV-1)) is the most likely, because all the rest specifically say &#8220;Gag-Pol polyprotein (Pr160Gag-Pol)&#8221;.  I think I grabbed the sequence and was in too much rush when I tried to work out which protein I was dealing with.</p>
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		<title>By: suicyte</title>
		<link>http://www.iayork.com/MysteryRays/2007/06/14/epitopes-and-microsoft-computational-biology/comment-page-1/#comment-27</link>
		<dc:creator>suicyte</dc:creator>
		<pubDate>Wed, 01 Aug 2007 15:56:30 +0000</pubDate>
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		<description>Ian, are you sure that your test peptides come from a Nef protein? I am no expert, but they seem to be derived from a Gag-Pol instead.
Maybe you could post the Accession number of your input protein, this would make it easier to compare your results with another method.
Thanks!</description>
		<content:encoded><![CDATA[<p>Ian, are you sure that your test peptides come from a Nef protein? I am no expert, but they seem to be derived from a Gag-Pol instead.<br />
Maybe you could post the Accession number of your input protein, this would make it easier to compare your results with another method.<br />
Thanks!</p>
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