Comments on: Non-lysine ubiquitination

By: Steve Bailie

Steve Bailie — Sat, 10 Jul 2010 22:28:45 +0000

Not just in viruses:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC270...

"Ubiquitylation on Canonical and Non-canonical Sites Targets the Transcription Factor Neurogenin for Ubiquitin-mediated Proteolysis"

By: Interesting papers, July07 edition « Suicyte Notes

Interesting papers, July07 edition « Suicyte Notes — Thu, 15 Nov 2007 11:31:20 +0000

[...] Ubiquitin-Binding Proteins from Ivan Dikic’s group. This paper probably qualifies as a ‘everything-you-know-is-wrong paper‘ as it breaks a quasi-dogma in protein ubiquitination. Just a brief reminder: ubiquitin gets [...]

By: Mystery Rays from Outer Space - Meddling with things mankind is not meant to understand. Also, pictures of my kids

Fri, 29 Jun 2007 19:45:47 +0000

[...] Non-lysine ubiquitination [...]

By: iayork

iayork — Mon, 25 Jun 2007 12:28:53 +0000

I donâ€™t think that this effect (if true at all) is very widespread. I have heard several people talk about mutating all lysines with the expected result of abolishing ubiquitination.

I certainly don’t think it’s universal, but I have heard from two people who have mutated all their lysines without eliminating degradation — one of them still saw ubiquitination, the other hadn’t tested. Neither had published, partly because they were sure they had done something wrong. So I’m sure there’s a publication bias here. Ted’s paper should be high-enough profile that it might drive some of the less confident people out of the woodwork. I’d guess that the next two years will give us a better idea how common this is. If no, or very few, instances are published in that time then I’ll be comfortable that it’s rare. Right now, I’m on the fence.

By: suicyte

suicyte — Mon, 25 Jun 2007 09:17:38 +0000

I don’t think that this effect (if true at all) is very widespread. I have heard several people talk about mutating all lysines with the expected result of abolishing ubiquitination.

One thing I am afraid will be happening now is people claiming that this non-classical ubiquitination by mK3 is happening because this family uses a “PHD-finger” instead of a RING-finger :-)

(in case you have not followed the RING vs. PHD discussion: the ligase domain of mK3/MIR1 family is *not* a PHD, although it has been claimed at one point. It is rather a slightly atypical RING-finger)

By: Suicyte

Suicyte — Sun, 24 Jun 2007 19:52:02 +0000

Ian, again, a great Post! Exactly the kind of thing I planned to do when I started my own blog. Now it turns out that the more I work about ubiquitin during everyday work, the less I feel like blogging about this subject. Good do see that other people fill this niche much better than I could ever do!

Cheers,
Kay